Propeptin, a New Inhibitor of Prolyl Endopeptidase Produced by Microbispora I. Fermentation, Isolation and Biological Properties

KEN-ICHI KIMURA; FUMIKO KANOU; HIDETOSHI TAKAHASHI; YASUAKI ESUMI; MASAKAZU URAMOTO; MAKOTO YOSHIHAMA

doi:10.7164/antibiotics.50.373

Propeptin, a New Inhibitor of Prolyl Endopeptidase Produced by Microbispora

I. Fermentation, Isolation and Biological Properties

KEN-ICHI KIMURA, FUMIKO KANOU, HIDETOSHI TAKAHASHI, YASUAKI ESUMI, MASAKAZU URAMOTO, MAKOTO YOSHIHAMA

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JOURNAL FREE ACCESS

1997 Volume 50 Issue 5 Pages 373-378

DOI https://doi.org/10.7164/antibiotics.50.373

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Abstract

Propeptin, an inhibitor of the prolyl endopeptidase isolated from the mycelium of Microbispora sp. SNA-115, is an atypical cyclic peptide antibiotic. It was purified by column chromatographies on silica gel and Sephadex LH-20 and high performance liquid chromatography using an ODS column. Propeptin has the molecular formula of C₁₁₃H₁₄₂N₂₆O₂₇ and consists of nineteen amino acids. Propeptin inhibited prolyl endopeptidase of the genus Flavobacterium competitively when Z-Gly-Pro-ρNA was used as a substrate. The inhibitor constant (Ki) was 0.70μM.

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