Reducing the Histopathological and Biochemical Toxicity of Aflatoxins Contaminated Soybean Using Ozone Treatment
Mohamed S. Shahat
Department of Food Science and Technology, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.
Ahmed Noah Badr *
Department of Food Toxicology and Contaminants, National Research Centre, Dokki, Giza, Egypt.
Ahmed I. Hegaziy
Department of Food Science and Technology, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.
Sherif Ramzy
Department of Food Toxicology and Contaminants, National Research Centre, Dokki, Giza, Egypt.
Mohamed Abdel Samie
Department of Food Science and Technology, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Aims: Soybean which was more frequently used in Egypt for edible oil production and meal for animal feeding was chosen as oil seed. The study targeted to reduce the aflatoxins toxicity, improving safety using natural treatment.
Study Design: The toxicity of aflatoxins on rat’s tissues and blood serum biochemical was studied to identify its hazardous effect.
Place of Study: Samples were collected from local stored area, Egypt, analysis were done in Food Toxicology and Contaminants Department, National Research Centre, between 2014 and 2016.
Methodology: The biological toxic effect of soybean containing aflatoxins was studied. Soybean seeds were contaminated by aflatoxin B1 at 50 μg/kg and fed by male albino rats. After 35 days of feeding the blood serum and liver and kidney tissues were collected and biochemically and histopathological investigated. The contaminated soybean was exposed to ozone at two levels of 20 and 40 mg/30 minutes to study ozone reducing or removal properties on aflatoxin toxicity. The aflatoxin residues were estimated along with biologically investigated of contaminated seeds which treated by ozone to investigate ozone removal aflatoxin toxicity ability.
Results: The results exhibited that the soybean containing aflatoxins have great hazard effect on all tested items of serum biochemical. The liver cells were damaged and necrotized and further the renal glomerular and tubules were more inversely affected by aflatoxin. Using of ozone, either at low or high doses, had a reasonable effect on reducing of aflatoxins at levels of 84% and 100% for 20 and 40 mg/30 minutes of ozone exposure, respectively.
Conclusion: The improvement of effect of soybean treated with ozone was also biochemically and histopathological tested. The better use of ozone in removal of aflatoxins was recorded for 40 mg/30 min, which resulted comparable of all tested serum biochemical and screening of liver and kidney tissues as that obtained by soybean control.
Keywords: Soybean, aflatoxin B1, ozone treatment, histopathological effect.